FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496755
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT900.00534411619.4023361
ACGATTC1108.2430075E-418.81658666
GATCTAG2500.001297832211.03943355
GAATCTA3602.1782249E-510.5414784
CGGACGA2300.00879991910.496654535
CGCATCT2800.00333649769.85433211
ACCCATG5959.5746145E-89.2759056
GTTGAAT5653.764435E-79.2719131
GTTAAGT3050.0060932019.1604471
GTAGATA3550.00235351438.7507092
GTTTATT8901.2187229E-108.632951
TAAGTTC3700.00343883838.3917423
CTTAGAT3850.0044070158.16411
GTTTAAT6502.8230443E-68.0594321
ATTAAGC4754.481391E-47.98719530
AGATTAA5856.3142856E-57.664736328
GGAAAAT13600.07.44707351
ACATTGA8909.861651E-87.3650374
GTTAAAC4800.0038663547.2758761
GTTTAAC4850.00427724757.20086671