FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496755
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAAT950.00735940418.3563561
GTCGAAA1200.001373313817.438541
CCGTTAT1000.00988991917.4385381
GTCCGTA2451.3882527E-817.082651
GCCGTAA1452.5063273E-416.837211
GTCGGAT2554.0619307E-715.0450131
GTCCGCT1750.001045624613.9508311
GTCCGCC1750.001045624613.9508311
GTCCGGG2851.4258421E-613.46132851
TGCGATT1800.001398828913.42322
GTCGAGG2102.7329184E-413.2865061
CCCGTTT2701.2084431E-512.9174361
GTCCAGT7800.012.5199771
CCCGGTT2050.003436480611.90924551
GGCGTTT2651.4523123E-411.8450451
GTCCGAC2651.4523123E-411.8450451
GTGGTTT11000.011.731381
GTGCGGT3206.873016E-510.8990871
GTCCGGA2250.00687970610.8506471
CTCGTGT2903.3532738E-410.8239211