FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21873728
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT21550.015.540321
TCGACCG13650.014.90820631
CGACCGT14750.014.26411632
GTTCGAC16150.012.60092429
GTCCGGA29400.011.6282611
TCGACGC20100.010.81133443
CGCGTAA8350.010.74143767
GTCCGTT28000.010.7146121
TCGCGTA8750.010.64501166
CCCGAAT24750.010.5711461
GTCCGCT27550.010.3831291
GTCGTAT23200.010.3752161
GTCGCTT33300.010.16163351
AAGTTCG21400.010.15429227
TCTCGAC22050.010.011662541
TTCGACC20450.09.95095430
GTCGATT18950.09.9407821
GTCGCAT18100.09.8294131
CTCGTAT31650.09.8096231
CCCGTAT19800.09.6902181