FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24990266
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT24550.07.02489149
GTTTAAT124050.06.94912241
CGGGAAT35550.06.67573451
GTTGAAT101650.06.55776361
CGGTACA30900.06.475640361
GATTCCG27550.06.38565252
GTTAAAT122450.06.38438371
GTTTTTT351250.06.37893871
GGAAAAT245250.05.99104831
GTTTATT162250.05.97984361
AATCGTC27950.05.8020725
GGGAAAT156400.05.77953341
GAATCGT32900.05.7682074
TCGATTC30500.05.65518550
GGCGGTA31900.05.623466559
GTTTGAT148100.05.5378681
CTTTAAT149350.05.5148861
TTCTCGA31950.05.506493647
CGCGGTA10659.0543996E-55.50556817
TCTCGAT31350.05.50184448