FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24990266
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT31400.013.4408281
GTCCGCT32900.012.1919241
GTCGGTT21750.012.0274151
GTCGCAA11750.011.5770271
GTCGTTT44350.011.3250261
GTCCGAT24800.010.8295231
GTCCGAA24950.010.4848211
TCGACCG9800.010.20654231
GTCGGAT28600.010.0004181
CCCGAAT29000.09.8624811
GTCCGGG49600.09.8450211
GTCCGTA30950.09.8045791
GCCCGTT48600.09.760521
GTCGAAT19250.09.60318851
GTCGCAT21400.09.4533231
GTCGAGT21500.09.2471251
GTCGGTA24200.09.2243321
CCCGTAT23650.09.14388751
GTCCAGT124400.09.0282961
GGCGTTT41750.09.0227221