FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4457028
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA6350.014.12580761
GATTCCG5805.293259E-1010.70556152
CTCGATT5402.1464075E-810.21980149
GCGTAGT4159.563428E-69.97309318
TCGATTC5857.385643E-89.43514350
CGATTCC6951.204171E-99.43047851
GTCTAAG8557.2759576E-129.27954444
GGCGGTA8607.2759576E-129.22621459
TTGCGTA4906.831391E-69.15058516
TCTATCG3200.0099759288.6264413
GCGGTAC9609.094947E-128.62489260
TCGTCGG4805.316314E-58.62431148
CGGGGTT6402.9384682E-78.6225711
TCTCGAT6051.2083638E-68.55303648
GGTTGCG5502.9304878E-58.15215614
CGTAGTG6909.213236E-77.99774519
GCGTCGT5201.3556005E-47.95929519
TTAAGCC13150.07.868151731
CGAACGA3950.0062030137.86013247
TAGACGA4400.00186133827.84028347