FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4457028
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2255.5624696E-918.39533831
CGACCGT2451.5950718E-816.89367932
GTCCGGA5550.013.8226261
CCCGTAT3052.0499465E-713.7196251
TCGACGC3204.1960266E-712.93523943
GTTCGAC2952.3808752E-612.86215829
TCTCGAC3801.9075742E-812.70830541
GTCCAAT13100.012.24467851
CCGTATT5950.012.183092
CTCGTAT4702.064553E-911.8708811
GTCGCTT5652.1827873E-1111.7264351
GTTCGAT7650.011.39565851
GTCGCCT4004.4077024E-711.3329821
GTCGCAT3853.4260174E-610.8687941
GTCGCAA2250.00689269110.8486671
GCGATCG3207.697234E-510.77936640
GTCCGCT5502.0991138E-910.7782211
GTCGCTA4551.999033E-710.7294511
GTTCGGA3903.9984407E-610.7294511
CGGATTG4854.8283255E-810.6751843