FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324977
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA2202.8168419E-514.11164446
CGCATAA2506.12063E-613.79864647
TTAATCG1800.001402915913.4185253
CGGTACA3355.0333256E-813.38760961
GCGTATT1850.001583391213.204741
GGCGGTA3701.8428545E-712.12043159
GTCTAAG3702.9541194E-510.25486744
AATCGAA3250.0010564799.5551695
GCGGTAC4351.6655362E-59.51692160
ACGGGCG5452.6717498E-79.4944856
CGAAAAT3400.00143844519.237771
CCCGGAT3150.0079918698.8630461
GGGCGGT5452.7221267E-68.86189658
ACACACG5755.6161643E-68.39863352
TCAATCG4957.595449E-58.3647953
GCTTGTC6402.7065835E-68.08392617
CAACGCG3900.00553492647.960242749
GTTTTAT19100.07.8566721
CAATCGG4000.0069271977.76357464
GTTAGTT6301.6322587E-57.7551651