FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324977
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT1605.077527E-821.8083971
ATATGCG850.00412751120.288956
CGATCTA900.005744590419.1617854
TAACGGG1351.5798531E-417.88587242
TACGACG1753.2171447E-617.7391039
CTACTCG1653.8527192E-516.7230134
CCGATAT1250.001937465916.5650463
CCCGACT2653.6334313E-815.8008011
TCGACCG1554.5353695E-415.57667831
CCGGAAA2251.8216706E-615.5081941
GTCCGAA2051.2970511E-515.3190691
GTCCGCT3251.8007995E-915.0310181
GTCGCAT1901.16453855E-414.6919741
CGAATTC2353.2107928E-614.6853243
CGACCGT1709.138089E-414.20226532
TCCGATA2708.730076E-714.0613512
TTACCGC2001.9871173E-413.79648538
GTCGGCA4405.0931703E-1113.4815551
CCGAATT2052.4358569E-413.4689662
TACTCGT2355.243262E-513.2094015