FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22487156
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTA30300.07.4003359
CTCGATT24350.07.365899649
CGGGAAT34100.07.2691551
GATTCCG28100.07.119965652
CGGTACA32950.06.805491461
TCTCGAT27350.06.68491748
GTTTATT137200.06.2598071
GCGGTAC36700.06.20408260
GTTTGAT130450.06.1287421
GTTTAAT106750.06.01769161
GGAAAAT213650.05.98078441
TCGATTC31150.05.98015150
ATTCCGT29950.05.873940553
CCGCTAG11802.0350471E-65.84748265
GTCCGAT12557.945873E-75.8419191
TCGCGAA8306.9725147E-45.817722333
TCACCCG40850.05.659213
TTCTCGA32400.05.64297747
CAATCGG22650.05.6364624
GTTGAAT92500.05.623731