FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22487156
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG11150.012.9921831
GTCCGAT21950.012.5558261
CGACCGT12500.012.41683932
GTCGGTT22000.012.0515711
CCCGTAT19700.011.6877371
GTCGTTT41800.011.43396951
GTCCGCT29500.011.3527741
GTCGGAT24200.011.1001311
GTCGATT19350.010.9976941
GTCGTAT24600.010.7778271
GTTCGAC14250.010.65037229
CCCGAAT24500.010.5370351
GTCCGTT28200.010.1441931
GCCGTAT20300.010.13931751
CGCGTAT4501.997054E-610.0782151
GTCGAAT18250.09.9401561
GTCCGGA30550.09.9348381
GTCGGCT38100.09.888981
GTCGCAT16650.09.8477351
TTCGACC16100.09.42621430