FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22377837
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG13550.015.01832531
CGACCGT15300.013.30057232
GTCGCGA5255.456968E-1212.6225731
GTTCGAC17650.012.31187129
GTCGGTT19800.012.1545121
CGATCGG16100.011.35509741
GTCCGCT30100.010.8921831
GTCCGCA30900.010.8359351
CCCGTAT20850.010.7060071
TTCGACC19050.010.68234930
TCGCGTA10800.010.54099266
GTCCGTT26550.010.5094271
GCGATCG17250.010.39810240
CGCGTAA10950.010.39624667
TCGACGC21300.010.364305543
GTCGAAT18250.09.9378871
GTCGTTT36150.09.9376231
CCCGAAT22950.09.8783461
GTCGGTA20700.09.7726251
TCTCGAC23100.09.55671941