FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15454881
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG8750.014.97888331
CGACCGT9500.013.43327832
GTTCGAC10100.012.97755229
CCCGTAT13850.011.8374421
GTCCGAT17700.011.6275591
GTCGCTT21600.011.4660651
GTCGGAT16950.011.3188631
GTCCGTT19150.011.2936081
TCGACGC12900.011.23026943
GTCGTAA13550.010.5549061
GTCCGCT21250.010.1775331
GTCCGGG31950.09.9352861
GTCGATA8850.09.8538641
TTCGACC13100.09.74169130
GTCCGGT23200.09.62280751
AGTTCGA14000.09.60875628
GTCGAAT12450.09.5261931
CTCGTAT23250.09.3020471
GTCGGTT13650.09.1998271
AAGTTCG13550.09.16418427