FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14393520
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT19200.012.57509949
TCTCGAT20350.011.86595248
GATTCCG21900.011.49803352
TCGATTC22300.011.29237850
ATTCCGT24200.010.26270753
TTCTCGA23750.010.16724747
CGATTCC28550.09.30316951
TCACCCG29800.09.1468873
GGCGGTA23000.08.84953259
CGGTACA22950.08.71894761
CGTGGGT31450.08.66591957
CCGGACA29050.08.31076212
GTCTAAG26000.08.22548844
TTTCTCG30500.08.03005346
CCCGGAC31050.07.997603411
ACCCGGC38650.07.67729855
CGCTAGT6452.6129E-57.48625933
GCGGTAC27750.07.459453660
TAGCATA40850.06.5854049
ACGGACA42100.06.553946518