FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14393520
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT12650.015.9961431
GTCGCAT11500.013.045131
TCGACCG9650.012.86714931
GTCCGTT18500.012.8237481
CGATCGG9800.012.67073141
GTCCGAT14850.011.9817861
GTCCGCT18350.011.7878191
CGACCGT10600.011.71400332
GTCGGAT19000.011.56817151
GTCCGAA14850.011.5119111
GTTCGAC11450.011.44734729
GCGATCG10850.011.44453140
GTCGAAA9550.011.3249451
GTCGAGA9850.010.625831
GTCCGGT21900.010.5142341
GGCGTAT14350.09.96803951
GTCGTTT22550.09.9017331
TCGACGC14050.09.81987343
GTCGGGT20300.09.7961771
TTCGACC13050.09.77908830