FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5535687
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAT7051.5097612E-109.78616248
CGGTACA7454.1836756E-119.72517361
CGCTAGA3209.1840094E-49.70293866
ACTAGGC8950.09.63355211
GGCGGTA8102.1827873E-119.37010559
CTCGATT6302.331035E-89.30690649
TCGTTGA9700.09.246325552
CCGTTAG3100.0077164898.90144639
CGATACG7759.131327E-108.90023929
TAGCCGA3905.640718E-48.84438563
CTCGCGA3200.0099998858.62397745
CTAGGCT10801.8189894E-128.30269512
TACGATA8454.62569E-98.16294227
GCGTAGT3900.0055441667.95915618
ATTCCGT8252.6000635E-87.944512453
GATTCCG7453.4731966E-77.8715552
TACGGAA10102.5283953E-107.853942432
CAACTAG12400.07.7875699
CTAACGG6806.4110827E-67.60863564
AGACGCC17850.07.535355623