FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5535687
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG3051.8189894E-1218.09329231
CGACCGT3257.2759576E-1216.97985632
GTTCGAC3753.6379788E-1215.63632429
TCGACGC3808.367351E-1114.52316543
CGATCGG3202.7688657E-814.01271141
GTCCGTT7200.013.5691751
GTCGCAT4601.05501385E-1012.8949291
GTCCGCT7150.012.688061
GTCGTTA4407.221388E-1012.6880591
GCCGTAA4154.105459E-912.6116251
CGTGCGA4154.8748916E-912.46672437
GTCGGTT4204.9058144E-912.4614861
TCTACCG2255.376894E-412.2637847
CGGCTAT4003.3251126E-812.2122571
TTAGGCG5701.8189894E-1212.10416761
GTCGAAT5257.6397555E-1111.9630281
GTCCGGT9450.011.8153361
GTCCGAA5652.1827873E-1111.7336471
CCGGCTA5351.2914825E-1011.6045437
GGCGTTT9250.011.3163781