FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5342203
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT6100.013.00542749
TCTCGAT6700.012.35696548
CGGTACA7900.011.35413761
ATTCCGT7350.010.79432753
GATTCCG7201.8189894E-1210.540112552
GTAGTCG8900.010.46592457
CGATACG7403.6379788E-1210.25351729
TCGATTC7453.6379788E-1210.18689350
TAAGCGC3401.424421E-410.14394820
CGCCTAT5151.176395E-710.04546411
GGCGGTA10050.09.95450159
TCACCCG10300.09.7147973
GTCTAAG9350.09.59189844
TTCGGCG10150.09.51557138
CGATTCC9300.09.27291351
CTAGGCT10950.09.13418512
ACCCATG24250.09.1048356
TCGACGT3050.00673472749.0503033
GCGGTAC10700.09.02780460
CGGAATG11500.08.998672553