FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5342203
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA3656.039045E-1014.33581351
TCGACCG3152.2526365E-814.23439931
GTCCGAA5750.013.9535251
CGACCGT3304.1492967E-813.5873832
GTCCGGG9000.012.4031341
GCGATCG3451.0437816E-611.99767140
GTTCGCT6700.011.4543871
GTTCGAC4259.0563844E-811.36239329
GGCGACT9400.011.1331321
CCCGTAT4106.035898E-711.0607211
AGTTCGA4703.0733645E-811.00839828
GTCCGTT6351.6370905E-1110.9870281
ATTCGAA4451.7107959E-710.8514198
CGATCGG3501.6005157E-510.84075341
GCCCGAT5201.0006261E-810.7334811
GTCCGCT6201.2369128E-1010.6902011
TCGCGTA2650.00211085810.41443766
GGCGTTT8450.010.3206551
GTCGGGA7800.010.2862531
CGACTAC8750.010.2573113