FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9816450
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG40900.054.75273
CGTATGC43950.053.453991
ATGCCGT44400.048.8046074
TGCCGTC50400.042.309055
CCGTCTT43350.041.6106267
GCCGTCT48000.039.961776
GTATGCC59300.039.3559882
CGTCTTC60550.029.334768
GTCTTCT77650.018.477239
TCTTCTG128750.010.3131110
TTACGCG4351.4921734E-610.30984155
TACGCGA4703.6212805E-710.27630356
CTCGATT11600.09.81217649
CTTCTGC119400.09.64787311
TCGATTC13850.08.96638550
GGCGGTA15150.08.88061959
CGGTACA15500.08.68048761
ACGCGAC5603.931922E-68.62479857
TCTCGAT12150.08.51749448
ATTCCGT14100.08.31776853