FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9816450
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTT14950.013.7669631
TCGACCG5850.012.9710631
GTCGGAT11100.012.5708411
GTCGTAT10700.012.3887411
CGCGTAA3357.3105E-712.3566467
GTCCGTT11900.012.312031
GTTCGAC6550.012.111917529
CGACCGT6600.012.01973232
TATGCCG6050.011.9808873
TCGCGTA3501.2403234E-611.82767266
GTCCGCT13000.011.5385821
TTCGCGC3702.4260153E-611.1864597
GCCGTAT11300.011.1135141
GTCGTTT16700.011.0709971
GTCGTTA8100.010.7666921
GCCGGTT10700.010.7586431
CGCGTAT2300.00809257810.6168951
GTCGGTT10200.010.6020251
CGATCGG6553.6379788E-1110.53247841
GTCGCGT5159.966425E-810.1604131