FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687692
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG4351.2471355E-711.10201744
GTTAGAT6351.8735591E-1010.4433431
GGCGGTA4201.0995973E-59.85623959
CGGTACA4251.26488085E-59.74064661
TAGATTC5503.0428237E-79.4099793
GTCTAAT4552.482207E-48.4380271
GTTTAAC9353.4015102E-108.2124121
GCGGTAC5151.2079214E-48.03839760
TTTTCGG4000.0069421987.761788454
GAAATCG5109.553918E-47.44187933
ACGGGCG5653.5254387E-47.327034556
CAACGAT4850.0043056317.1964431
GTTTGAC10251.7329512E-87.15080741
GTTCAAT7855.3385684E-67.11393641
GTTTAAT13809.094947E-127.08171751
TTAGATT9603.6497295E-76.8342392
GTAGATA7109.105008E-56.8089082
GTTTTAC14151.1277734E-106.6598891
TAAGTAC7301.3257415E-46.61583247
CTTAACT10056.7039036E-76.598531