FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687692
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATT2602.8987415E-816.0938381
GTACGAT2906.548362E-915.6313711
TACGACG1657.2901824E-414.6326039
TCGACCG1450.005226193514.27219437
GCCTACT4505.456968E-1213.9479921
GTCCGTT3659.320502E-913.3747861
GCGACTA1600.00996850812.9450132
GTCGGGT3902.3548637E-812.5174291
GTTCGGT3407.6680044E-712.3070521
CGATTTA2255.376297E-412.2635154
CTCGTCT5903.6379788E-1211.8203321
GGCGTTT3651.8051142E-611.4641031
GTCCAAT8050.011.2623551
CCCGGTT3752.49806E-611.1583941
GTCGAGT2500.001187178211.1583931
GCCATAT7900.011.0348041
GGCGTAT2852.8616987E-411.0115731
GTCGAAT2550.00140072310.9396021
TCGTCTA2853.1418298E-410.9010632
GCCGTAT2250.006892553510.8484391