FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255549
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT2505.0931703E-1119.31517649
TCGATTC2502.0414518E-816.5580750
TATTCGC1400.00414056314.7813677
TAAGCGC1550.0081294613.35091320
GATTCCG2901.96182E-613.08525952
TCTCGAT3357.299295E-712.35704148
CGAACTA2000.003097671512.0760013
ATTCCGT3401.1583708E-511.16095853
TTCTCGA3752.841838E-611.03895747
TCGCCTA2250.00747659810.73017810
TAAAGCG3055.921826E-410.1773358
CCGGACA4754.1950625E-710.16565812
CGAAAGT2750.002871987410.03474751
CGATTCC3803.960395E-59.98523451
AATCCGT3000.0058801899.2005595
CGTGGGT4603.2166867E-59.00074757
TCACCCG4653.6529433E-58.9039643
TTGAATC7801.0095391E-98.8515992
TTTGACG3150.0087353338.7672992
GTTCAAT7503.6508936E-88.3690361