FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255549
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT2650.024.9960381
CCGGCTA1356.844619E-620.43874437
CGACCGT1452.7301366E-416.65015832
TCGACCG1452.7301366E-416.65015831
GGCGTTT3501.8189894E-1115.9373231
GTTCGAC1554.5315453E-415.57837229
TCCGTTA1901.2729877E-414.5390252
GTTCCGT2454.451478E-614.2297541
GTCGCTT2505.4870197E-613.9451581
TCCGCTA1500.006493681613.8120742
TCGACGT1500.006540592813.7970759
GTCCGAT2901.742841E-613.2238571
GTCGTTT3701.1306838E-813.1913671
CCGTAAT2508.466811E-512.5506421
GGCGTAT2902.5289819E-512.0216881
AAGTTCG2050.003732619811.778769527
CTCCTAT6850.011.70579051
TTTCCGC2953.3623506E-511.6916537
GTCAACG2408.448533E-411.6209651
TAGACGC2100.00447314311.49679339