FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10068588
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT12050.09.15986549
CGGTACA15350.08.98986761
GATTCCG14350.08.89414152
ATTCCGT14450.08.59387253
GTAGTCG10254.0017767E-118.077764557
TCGATTC14700.07.97863850
GTCTAAG18750.07.910700344
GGCGGTA17050.07.68853659
TTCTCGA14950.07.61451247
TCTCGAT14600.07.560777748
GCGGTAC19450.07.44957360
CGCTAGT4750.0039472167.26135233
ATCGGAT13555.2750693E-116.8741616
GCGCTAG5050.0071573556.82971332
CAATCGG15751.8189894E-126.79141864
CCGGTAA7653.3569304E-56.76282268
TAGTCGC13352.6739144E-106.718865458
TCACCCG18700.06.64263253
TCTAACG6250.00111938286.6221557
GGAAAAT94050.06.5679941