FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10068588
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4650.018.54370931
CGACCGT6100.014.13584932
GTCCGAT12800.013.8990311
GTCGGTT8800.012.6850341
GTCCGTT11450.012.49115851
GTCCGCT11800.012.4162841
GTTCGAC7300.012.28518329
GTCCGAA10100.012.0884611
GTCGCTA8150.011.9846331
GTCGTTT15450.011.740841
CCGGTAT9850.011.3328221
GTCGTAA9000.010.4651531
TCGACGC8900.010.46453743
CCCGAAT12750.010.3967531
GTCCGGA13450.010.3743771
CCCGTAT8150.010.2725431
TTCGACC8500.010.1445530
GTCGCAT6201.3897079E-910.1275671
GTCGCAC14850.010.1010461
CCCGAGT10550.09.9195771