FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22720475
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT26100.09.77928849
GATTCCG29750.08.81240152
CGGTACA34600.08.47513961
TCGATTC31250.08.05845850
TCTCGAT30000.08.04925848
GGCGGTA33300.07.87328759
GCGGTAC38100.07.78711360
ATTCCGT34750.07.5444353
TTCTCGA33250.07.469989347
GTTTAAT105250.06.96205431
GTCTAAG41950.06.742736344
CGATTCC40300.06.67655551
TCACCCG44550.06.66080053
CGGGAAT32550.06.64631461
CCGGACA43350.06.603663412
TTTCTCG42650.06.38957446
GTTGAAT93100.06.1840711
GTTTTAT177250.06.1222961
GGGAAAT138450.05.97304251
TCGCGAA10409.94065E-65.96946133