FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22720475
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16000.011.85632432
TCGACCG15500.011.79371331
CGCGTAT3702.126988E-611.3097841
GTTCGAC18150.010.26229629
GTCGTTA17950.010.1021471
GTCGGTT23350.010.0060511
CCCGTTT56550.09.92814351
GTCGTTT37950.09.8321121
CCCGTAT19600.09.6075471
GTCCGTT24750.09.5809491
GGCGTTT38800.09.5268421
GTCGGAA32300.09.5006861
GTCCGAT25100.09.44735051
GTCGAAT17400.09.4194041
TTCGACC20000.09.31262430
CCCGAAT24950.09.0848471
TCGCGTA9500.09.07862966
GTCGGAT25750.09.0734481
CCGGTAT22850.09.00410651
CCGGAAA20200.08.9769091