FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25129111
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT27150.08.51173249
GATTCCG33650.07.58602252
TCTCGAT32350.07.57107548
CGGTACA37350.07.48172361
GGCGGTA34600.07.178694259
TCGATTC34750.07.048180650
GCGGTAC41800.06.685183560
TTCTCGA37400.06.548777647
GGAAAAT243700.06.4008431
GTTTAAT119450.06.3103411
CAATCGG24100.06.15646274
TCACCCG46450.06.09127143
GTTTATT159050.06.0775881
GACGTAA17602.0008883E-116.07529341
GTTTGAT148600.06.0353091
ATTCCGT37550.05.97132753
CCGCTAG12155.4223347E-75.96304965
GTTTTAT195750.05.91863441
GTTCAAT66850.05.89878031
TACGCGA8802.304982E-45.880474656