FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25129111
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG14150.014.38138131
GTTCGAC16800.012.72940629
CGACCGT16600.012.25885132
GTCGTTT43750.011.7175591
GTCGGTT23650.011.0593091
GTCCGTT31700.011.00116351
GTCGGAT27900.010.3746091
TCGACGC20750.010.1401743
TTCGACC21150.010.11085830
GTCGGAA31450.09.8688651
GTCCGAT26200.09.7167141
GTCCGCT30900.09.7059461
CCCGTAT22550.09.5883311
GTCCGGT35200.09.5110051
GTCGCTT35200.09.4119331
CCCGAAT29900.09.3307521
GTCGGCT39500.09.2702211
AGTTCGA23500.09.24694828
CCGGTAT25650.09.2452661
CGATCGG16600.09.142776541