FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3926529
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG4101.8189894E-1215.14334252
CTCGATT4255.002221E-1012.98467149
CGTAACG2409.251849E-411.49611130
TCTCGAT5006.4592314E-911.03837748
ATTCCGT6009.549694E-1010.3479553
TCGATTC5755.2186806E-910.198550
TTCTCGA5907.963536E-99.93921647
GTTGACG3050.00611185759.1577241
TAAGCGC3050.00675614049.04669720
CGATTCC6554.3766704E-88.95265351
CGATACG3650.00305263638.503756529
CGTGGGT7851.07193046E-88.3499457
TACATCG8353.6725396E-98.2641523
CCCGGAC7554.9276423E-88.22290811
CAACCGA5101.0770133E-48.1183144
ATCGACT5553.270388E-58.0809226
GCGTCGT4400.00186478567.838757519
TTTCTCG7951.17577656E-77.809972346
TTTACGG4000.00693678257.762643362
TCACCCG8252.1800588E-77.52789163