FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3926529
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2152.2713313E-514.43800231
CCGGCTA2901.9660529E-613.08337637
CGACCGT2509.363757E-512.41668132
CCCGTAT3407.6091055E-712.3153011
GTTCGAC2852.3629393E-512.10218229
CCCTAGT2357.0307544E-411.8785881
GTCCGTT5308.913048E-1111.8505721
TAGACGC3257.0280257E-611.67469439
CGGCTAA3551.4733941E-611.6594538
CGGCTAT2701.7221778E-411.6311171
GTCGTTT6351.8189894E-1211.5395331
GTCGAAT4309.073119E-811.3606251
GTCGTAT4702.591878E-811.1361761
CCCGATT3155.833676E-511.0772541
CTCGTAT5401.5334081E-910.9849441
TCGAATA3156.504565E-510.9593632
TCGGATC4251.0965177E-610.5596692
TTCGACC3301.0524291E-410.45188530
GTTCGAT6351.891749E-1010.440531
TCGCATA2650.002098388510.4217342