FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800548
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT1052.1026888E-523.244321
CGGTCGT1300.002524617815.92103255
GTTTAAT7900.011.9163921
CTCGATT2100.004468449411.49820449
CGGTACA3157.9393695E-49.8575261
CGTGTAC2850.00385888229.68269846
TACATCG3250.00105586079.5555393
GGAATCG3300.00121405039.4107583
ATCTAAC2950.00512414759.3555116
GTGAGAC4804.4643466E-47.99023441
TCACCCG3950.00618511677.8621533
ATCGATG4450.00207793347.75245956
GTTGGAT8101.3413046E-77.74810651
GGAATAT7704.94676E-77.69779351
TACTAAC4500.00232022377.66589548
CGGCACT4500.0023246567.664404423
GTTAAAT8201.6482409E-77.6536171
TACAATC4600.00285697457.50487382
ACACACG4150.0096515757.480551252
GGAAAAT17450.07.3928921