FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800548
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1551.3587851E-924.7294031
GTCGAGA1755.5333658E-921.9031851
GTCGTGA1653.5283607E-516.895021
CCCGTAT1400.003866785214.933991
TCGGGTA1450.00520061114.2823882
ATTGACG1800.001406683213.4134816
CCGGAAT1850.001601119513.1849651
GTCGTTT3254.472604E-712.8662061
CCCGTTT4651.2914825E-1012.739391
GTCCGTT1950.002378594412.5088131
GTTCGGT2305.932876E-412.120341
GTTCCGA2050.003459966711.8986261
CTCGTAT2357.108107E-411.8624611
GTCCGAT2752.0671618E-411.4041381
GTCCGGG3702.1423948E-611.3013981
GTTCGAT3904.0281557E-610.7218391
GCCCTAT3258.120685E-510.7218391
CTCGTCT3954.6911246E-610.586121
CTCGTTT5602.8539944E-910.5782421
CCGTATT2300.00875062410.50482