FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3845142
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAT4150.0086784357.57060861
CTCGATT4700.00355856877.338653649
GGGAAAT23550.07.2634271
GTTAAAT16950.07.20833061
GTTTTAT30050.07.2025051
TCTCGAT4850.00482625657.11270248
GTTTATT24050.07.1124191
GTCAAGT12302.2919266E-107.09530831
TTAGATT13202.7284841E-117.06283042
GTTTTTT55750.06.8878581
GAATCCG5100.0078317216.76615244
AATCTAC11251.7886123E-86.74810935
CTCTAAT10401.6987542E-76.7132531
GTTTAGT11251.02081685E-76.5163311
GTTTGAC15108.0035534E-116.47317651
GAATCTA12502.1422238E-86.3493574
GTTAGAT8802.8788883E-56.3470761
AAATCCA30450.06.34618474
GTCTAAG6000.00530910076.32380244
GTCGGAA6100.00556692436.2950511