FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3845142
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT4800.018.1720431
TCGCATA1855.4205902E-616.79452
TCGACCG2502.0470907E-816.55527531
CGACCGT2657.128838E-714.316668532
GTCGGAT3901.07320375E-1014.3139771
GTCGGGT5450.014.0841661
GTCGTAA2405.736482E-513.0838711
GTCCGCG2456.9593436E-512.8168521
CCGGCTA1900.002113811412.707561537
GTCGACT3356.3663356E-712.4980251
TTCGACC3104.1530093E-612.23844230
GTCGCAT3459.0877074E-712.1357631
CCCGTAT4358.265488E-912.0311441
GTCCGGA4358.265488E-912.0311441
GTTCGAC3205.915761E-611.85660729
GTCGTTA3601.5187707E-611.6301071
GTCGGAA5105.9480953E-1011.6301061
GTCGATA2150.00489312411.3596391
TCGACGC3359.848054E-611.32586243
GTCCAAT12050.011.2923021