FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534695
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTA6600.011.49887559
CGGTACA8350.010.32867361
CTCGATT5502.8536306E-810.03394249
TCTCGAT5402.3400571E-79.58218448
GCGGTAC8455.456968E-129.38992560
CGGGAAT6051.03667844E-79.2295471
GTCTAAG9550.09.03003244
CAATCCA15350.07.8671554
GTTCGTT4905.518569E-47.83451941
CACGGGC12651.8189894E-127.635953455
CGCGGGT4100.0086741157.57109426
CCAGTAG11952.0008883E-117.5069495
GTTCAAT11252.4374458E-107.4451681
ACGGGCG10708.4946805E-107.415489756
TTCTCGA6653.903299E-57.26228747
GGGCGGT11606.148184E-107.13762658
TAGCATA13557.2759576E-127.12696089
GAATCGA4900.00532277677.04144764
TCTAAGT12759.094947E-117.03437345
ACACGGG12302.9831426E-107.011354454