FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534695
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT5850.014.9050091
GTCGCAT2951.3614044E-714.1875491
GTCGGTT4505.456968E-1213.9510911
CGTGCGA3506.031769E-913.79679537
GCGATCG3204.1967724E-712.93506640
CCGGTAT3902.3492248E-812.5202091
CGATCGG3408.748302E-712.17418141
GTCCGTT5206.548362E-1112.0730591
TCGAGTT4359.6988515E-911.9021892
GTCCGGA5601.8189894E-1111.8335131
GTCGGAT5055.0931703E-1011.7410161
CCCGAAT4802.8667273E-911.6259081
TCGCGTT2409.1896066E-411.5054492
CCCGATA3601.7294587E-611.5054492
CTCGTAT7700.011.3239361
GTCGCTA3752.492663E-611.16087151
CTCGGAT6900.011.1204341
CCGCGAT3155.8608006E-511.0722931
CCCGTAT4106.048722E-711.058791
GTCGCGT2852.8566463E-411.0140191