FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25961660
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT34150.011.10999749
GATTCCG34850.010.88801552
TCTCGAT36550.010.193192548
TCGATTC39850.09.86846450
CGGTACA40000.09.31474861
TTCTCGA42350.09.28592947
CGATTCC47050.09.16438651
ATTCCGT40450.08.95425853
GGCGGTA41450.08.40588159
GCGGTAC48850.08.05089760
GTCTAAG48950.07.822082544
TCACCCG51400.07.78747653
CCGGACA53500.07.671510712
CGCTAGA17800.07.36515466
ACTAGGC32900.07.337993610
CCGCTAG17400.07.33619165
CCCGGAC55550.07.26422911
TTTCTCG56350.07.0399246
CGGCCCG71950.06.6630428
GGGCGGT58700.06.64117858