FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9699926
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA16300.010.37127861
GGCGGTA17300.09.77127759
GTCTAAG19700.09.63049444
CTCGATT11700.09.43332349
CGATACG9051.8189894E-129.14615629
GCGGTAC20450.08.2665560
ACGCGAT4350.00166257977.93091658
TCTCGAT12750.07.846172348
GATTCCG14750.07.48366952
CATATGC27900.07.416949312
TCACTAA31000.07.3427798
ACTCACT35250.07.3407316
ACACACG24550.07.30648452
TACGATA11001.5279511E-97.21129727
TTCTCGA14850.07.20120647
AACTCAC35550.07.183295
CTCACTA27400.07.1747177
ACGGGCG24600.07.011981556
CGCGGTA5450.00194035566.9612617
CTAAGTA29450.06.910755646