FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26876580
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT31400.010.21580149
GATTCCG35900.09.12851352
TCTCGAT36050.08.2294948
TCGATTC37750.08.13300850
GGCGGTA40100.08.00070259
TTCTCGA39200.07.744197447
ATTCCGT39650.07.656148453
CGGTACA42550.07.54041961
GCGGTAC46850.06.995566460
CGATTCC46700.06.943438551
TCACCCG55400.06.9131023
GTTTATT169800.06.67856841
GGAAAAT258150.06.56903841
GTCTAAG49100.06.533630444
GTTTAAT120850.06.0633181
TTTCTCG52000.06.036740346
CGCGCTA12601.6902413E-76.02206523
CGTGGGT56750.05.957579657
CCGGACA53550.05.925465612
CCCGGAC56700.05.900418311