FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26876580
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG15750.015.11053431
CGACCGT17650.013.8748232
GTTCGAC19500.012.912683529
GTCCGTT31450.011.5310331
TTCGACC21700.011.44416430
CGCGTAA10450.010.89383767
GTCCGCT34250.010.8937831
CCCGTAT26850.010.7792691
GTCGTAT29100.010.5449671
TCGCGTA10500.010.51380866
GTCGGTT24250.010.4970341
GTCCGGT39450.010.3417591
TCGACGC24300.010.22019843
AAGTTCG25000.010.20986527
GTCGGAT29000.010.1003591
GTCCGAT28350.010.0859381
GTCCGTA31300.010.0266011
GTCCGGA37350.09.98961451
AGTTCGA26500.09.8922728
CCGGTAT23100.09.8119861