FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27000805
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT31150.010.62980849
GGCGGTA40200.010.38371759
CGGTACA44200.010.0688461
GCGGTAC49950.09.047960
TCGATTC41050.08.90774350
GATTCCG41350.08.84283752
TCTCGAT37050.08.75216448
GTCTAAG52650.08.58269744
TTCTCGA38950.08.50236247
ATTCCGT40700.08.39077553
TCACCCG50600.07.84210253
TAGCATA72150.07.64823689
ACGGGCG62350.07.414336756
CCCGGAC52900.07.30209111
ACACACG67450.07.26219252
CCGGACA51950.07.23645512
CGATTCC52750.07.12782951
GGGCGGT63950.07.067111558
CATATGC76050.06.847982412
TTTCTCG52100.06.48854446