FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27000805
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG16900.015.71480131
CGACCGT18200.014.59231532
GTTCGAC20150.012.66720629
GTCGAAT20650.012.5000791
GTCCGCT34100.011.8660111
GTCGCAT20100.011.6273271
GTCGGTT23650.011.2094321
TTCGACC24950.011.05923630
GCCGTAT24800.010.6896391
GTCGCGA6007.876224E-1010.4645941
CGATCGG19050.010.32080741
TCGACGC26950.010.23913943
AGTTCGA27050.010.07358428
GTCGTTT48000.010.0285691
GTCCGAT27850.010.01995851
GTCCGGA36700.09.9798581
GTCGGCT43000.09.9778691
CCCGTAT25900.09.966281
GTCGAGA17400.09.8230871
AAGTTCG27100.09.80044127