FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7936748
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA11650.010.65943461
GTCTAAG15300.09.24466244
GGCGGTA14000.09.11501459
TATACGC4502.5075907E-48.4305977
TAGCATA19350.08.0205029
GCGGTAC16450.07.967420660
AATCGAT8453.9770384E-87.75629765
ACGGGCG19150.07.744271856
GTTAAAT34950.07.5873521
GAATCGA8858.9790774E-87.4061044
GCATATG23650.07.291732311
CTCGATT8251.7462316E-67.10965249
CTAAGTA20450.07.084481246
CATATGC22750.06.973956612
CAGTCGA8101.0128237E-56.8141934
TTAAGCC23300.06.81161131
GGGCGGT20800.06.79844658
CTTAGAC11855.662514E-96.77225451
GTAGCAT26550.06.75474268
GATTCCG9956.927512E-76.58667452