FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7936748
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG3300.022.99223731
CGACCGT4000.018.10615732
GTTCGAC5300.014.31727429
GTCCGTT10450.012.6837741
GTCCGAT10550.012.5635471
TTCGACC6350.012.49223930
GTCGGTT6450.012.4379641
TCGACGC6700.011.84010743
CGCCTAT4309.125142E-811.3564011
CGCGATA2450.001099417911.26185822
CGATCGG6201.2732926E-1111.12674741
GTCGGAT10700.011.0834841
GTCGGCA15250.010.9787421
TCTCGAC7900.010.91548141
GCCGTAT7800.010.7324251
AAGTTCG7400.010.72036627
AGTTCGA7101.8189894E-1210.68754328
GTCGCTA6907.2759576E-1210.61576751
GTCCGCT10850.010.6087781
GTCGCAT6007.876224E-1010.4641131