FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5995551
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAT6950.016.37952648
CTCGATT7150.014.9575549
GATTCCG7150.014.9546852
ATTCCGT8350.012.80550453
TCGATTC8700.012.292750
CGATTCC9900.011.49731951
TTCTCGA9350.011.43593147
GTCTAAG11150.010.20931944
CGGTACA10750.09.94709961
GGCGGTA11800.09.93753159
ATTCGGT5058.294155E-79.6725121
TCACCCG13100.09.2161013
CGCGCTA3804.3576397E-49.07599723
GCGGTAC13100.08.95151460
CCGGACA12950.08.7893112
CGGGTTA3550.00237245148.74385743
ATACGCA6859.0563844E-88.5592638
CGTGGGT14100.08.31651757
ACGCGCG5907.963166E-68.18371320
TCGCGAA3800.00438751058.16894325