FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5995551
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA950.007784408518.1795982
CGACCGT4750.015.2474332
TCGACCG4600.014.99500831
GTCGGTT5600.014.9477111
GTCGCGA1708.401354E-414.36152651
GTCGCAA4151.8189894E-1114.2873711
GTTCGAC5100.014.2023429
GTCGCAT4458.6947693E-1012.5404021
TTCGACC6150.012.33787230
CCCGTAT4601.4679244E-912.1314751
CGCGTAA3154.9436585E-612.04923767
TCGCGTA3451.0404547E-612.00091266
TCGACGC5803.6379788E-1211.89358543
GTCCGTT8100.011.62599851
AGTTCGA6550.011.58489728
GTCGGGA10750.011.3556251
CTCGTAT7500.011.1609571
AAGTTCG7001.8189894E-1210.84015527
GCCTAAT7151.8189894E-1210.731691
CCGGCTA4552.3228677E-710.61335837