FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327448
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT4006.1514347E-610.34931249
TAATCGA3501.9032386E-49.8564874
TCTCGAT4704.0123923E-69.54163248
GATTCCG4704.1630094E-58.80580752
TTCTCGA4805.3198368E-58.6236547
GTTTAAT14650.08.3380351
AACGATC3750.00387345448.2824382
TCGATTC5059.6180374E-58.19747550
CGATTCC5958.908544E-68.11515651
TACGCCC4300.00148291048.02308569
GTTAAAT16600.07.9893011
ACGATCT4754.4772908E-47.98858263
ATTCCGT5301.6908381E-47.808923753
TTGATCG4100.008660317.5723263
GGCGGTA4650.0032054047.417524359
GTCAAAT9959.800715E-97.3659621
TCACCCG7151.2990606E-57.23695473
TCAATCC6704.307134E-57.20815133
TCTAATC8806.368409E-77.0588962
CACGAAT4950.00525497.0506321